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Rosetta Calibration

Rosetta Calibration sets a nanometer size gate on your flow cytometer.

Why use Rosetta Calibration?

You want to measure the size of your particles, but flow cytometry measurements are in arbitrary units. Use Rosetta Calibration to convert the arbitrary units of the light scattering signal to nanometers, know what you are measuring, and compare your measurements to other flow cytometers and laboratories.

Exometry calibration beads
Exometry calibration beads

How does Rosetta Calibration work?

Rosetta Calibration consists of beads and software. To set a size gate in nanometers, you need to:

  • run the beads
  • load the bead measurement in the software
  • apply the calculated size gate in nanometers to your own analysis software to select particles within the size gate


Rosetta Calibration is developed to standardize flow cytometry measurements on spherical particles <2000 nm, such as extracellular vesicles and liposomes. One vial of Rosetta Calibration beads contains sufficient material to calibrate your flow cytometer 25 times. We recommend to calibrate your flow cytometer weekly. If you change the settings on the scatter channels, you need to repeat the calibration. We currently support the following flow cytometers:

  • Apogee A50-Micro
  • Apogee A60-Micro
  • Beckman Coulter Astrios
  • Beckman Coulter CytoFlex
  • Beckman Coulter Gallios
  • Beckman Coulter Navios
  • Becton Dickinson FACSAria
  • Becton Dickinson FACSAria II
  • Becton Dickinson FACSCalibur
  • Becton Dickinson FACSCanto
  • Becton Dickinson FACSCanto II
  • Becton Dickinson FACSVerse
  • Becton Dickinson Influx
  • Becton Dickinson LSR Fortessa
  • Becton Dickinson LSR II
  • Stratedigm S1000
The vial also contains an activation code for the Rosetta Calibration software.


For more information or a quotation, please send an e-mail to info@exometry.com.

Coming soon

  • Track your flow cytometer alignment.
  • Use flow cytometry to size your nanoparticles.