Rosetta Calibration is an easy-to-use product that turns your flow cytometer into a particle size analyzer.
Why use Rosetta Calibration?
You want to measure the size of your particles, but flow cytometry measurements are in arbitrary units. Use Rosetta Calibration to convert the arbitrary units of the light scattering signal to nanometers, know what you are measuring, and compare your measurements to other flow cytometers and laboratories.
How does Rosetta Calibration work?
Rosetta Calibration consists of beads and software and works as follows:
you run the beads
you load the bead measurement in the software
the software automatically determines the scatter to diameter relationship for your particles and flow cytometer
the software adds a new channel "Diameter (nm)" to your flow cytometry data files
you apply size gates in nanometers using your favorite data analysis software
Rosetta Calibration is developed to standardize flow cytometry measurements on spherical particles <10,000 nm, such as extracellular vesicles and liposomes. One vial of Rosetta Calibration beads contains sufficient material to calibrate your flow cytometer 25 times. We recommend to calibrate your flow cytometer weekly. If you change the settings on the scatter channels, you need to repeat the calibration. We currently support the following flow cytometers:
Beckman Coulter Astrios
Beckman Coulter CytoFlex
Beckman Coulter Gallios
Beckman Coulter Navios
Becton Dickinson FACSAria
Becton Dickinson FACSAria II
Becton Dickinson FACSCalibur
Becton Dickinson FACSCanto
Becton Dickinson FACSCanto II
Becton Dickinson FACSCelesta
Becton Dickinson FACSVerse
Becton Dickinson FACSymphony A1
Becton Dickinson Influx
Becton Dickinson LSR Fortessa
Becton Dickinson LSR II
Cytek Northern Lights
The vial also contains an activation code for the Rosetta Calibration software.
The prices of Rosetta Calibration are:
€450,- for Rosetta Calibration beads + Rosetta Calibration software (1 year license)
€150,- for Rosetta Calibration beads
€300,- for Rosetta Calibration software (1 year license)
Prices are excluding VAT and shipping costs. Please ask for a quotation via firstname.lastname@example.org.
Download and install Rosetta Calibration and use "Demo" as a licence key to calibrate our demo files.