Rosetta Calibration

Rosetta Calibration is an easy-to-use product that turns your flow cytometer into a particle size analyzer.

Why use Rosetta Calibration?

You want to measure the size of your particles, but flow cytometry measurements are in arbitrary units. Use Rosetta Calibration to convert the arbitrary units of the light scattering signal to nanometers, know what you are measuring, and compare your measurements to other flow cytometers and laboratories.

Rosetta Calibration beads

How does Rosetta Calibration work?

Rosetta Calibration consists of beads and software and works as follows:

  • you run the beads
  • you load the bead measurement in the software
  • the software automatically determines the scatter to diameter relationship for your particles and flow cytometer
  • the software adds a new channel "Diameter (nm)" to your flow cytometry data files
  • you apply size gates in nanometers using your favorite data analysis software


Rosetta Calibration is developed to standardize flow cytometry measurements on spherical particles <10,000 nm, such as extracellular vesicles and liposomes. One vial of Rosetta Calibration beads contains sufficient material to calibrate your flow cytometer 25 times. We recommend to calibrate your flow cytometer weekly. If you change the settings on the scatter channels, you need to repeat the calibration. We currently support the following flow cytometers:

  • Apogee A50-Micro
  • Apogee A60-Micro
  • Attune NxT
  • Beckman Coulter Astrios
  • Beckman Coulter CytoFlex
  • Beckman Coulter Gallios
  • Beckman Coulter Navios
  • Becton Dickinson FACSAria
  • Becton Dickinson FACSAria II
  • Becton Dickinson FACSCalibur
  • Becton Dickinson FACSCanto
  • Becton Dickinson FACSCanto II
  • Becton Dickinson FACSCelesta
  • Becton Dickinson FACSVerse
  • Becton Dickinson FACSymphony A1
  • Becton Dickinson Influx
  • Becton Dickinson LSR Fortessa
  • Becton Dickinson LSR II
  • Cytek Aurora
  • Cytek Northern Lights
  • Stratedigm S1000
The vial also contains an activation code for the Rosetta Calibration software.


The prices of Rosetta Calibration are:
  • €450,- for Rosetta Calibration beads + Rosetta Calibration software (1 year license)
  • €150,- for Rosetta Calibration beads
  • €300,- for Rosetta Calibration software (1 year license)
Prices are excluding VAT and shipping costs. Please ask for a quotation via


Download and install Rosetta Calibration and use "Demo" as a licence key to calibrate our demo files.


Please see our publication list to read more about the background and applications of Rosetta Calibration.